PhenylEthyl Alcohol agar

PHENYLETHYL ALCOHOL:
*selective for gram +pos.
-inhibitor- phenylethyl alcohol inhibits gram -neg
interferes with DNA synthesis
divided the agar into 3 sections and streaked each section with a diff organism
E. coli- no growth, E. faec-growth clearing B hemolysis, S. aur- growth some white

Columbia CNA

COLUMBIA CNA :
*agar to promote gram +pos organism growth
-Inhibitor: antibiotics colistin nalixic acid CNA inhibits gram -neg growth
good growth is clearing-B hemolytic
greening good growth- a hemolytic
no growth same color - Y hemolytic

Methyl Red & Voges Proskauer Test

METHYL RED: (M.R.)
*M.R. part of test is used to detect organisms capable of mixed acid fermentation.
-medium contains peptone & glucose with a phosphate buffer and pH indicator
Indicator: Methyl red dye
* the solution turned red with a +pos result and yellow is -neg.
VOGES- PROSKAUER: (V.P.)
*for organisms that can ferment glucose and convert acid products to acetoin & butanediol
we added vp reagents after incubation it turned red for pos+ result
we used E. coli & E. aero.
following incubation we transferred part broth into two other tubes for VP test
E. coli was +pos for MR & E. aero was +pos for VP.

Phenol Red Broth Test

PHENOL RED BROTH:
*used to detect organism's ability to ferment in after a carbohydrate is added to test.
-indicator: Phenol red dye
-pH rises color turns pink is deamination of perptone and a neg.- result for carb hydrolysis.
-pH lowers it turns yellow a pos+ result for mixed acid fermentation
*3 tubes were used with phenol broth & durham tube for detection of gas production.
-inoculated with 3 organisms./ 24 hrs incubate
- A. faec,-no fermentation remained red
 E. faec-pink broth for peptone usage
 E. coli-yellow broth with a bubble in tube +pos for ferm

Tryptone test

TRYPTONE TEST:
*checking for the enzyme cysteine desulfurase a sulfur reduction enzyme. also thiosulfate reductases
for bacteria producing indole is SIM medium is used for. this test is a series of tests along with Indole, Methyl Red. Voges-Proskauer, and Citrate
*we stab inoculated 2 broths with a needle and incubated for 24 hours
we used E. coli & E. aero
*examined the stabline for spreading and black precipitation in the medium
the E. coli changed color and broke down indole to pyruvate is +pos for enzyme
the E. aero is -neg for enzyme no color change

Citrate test

CITRATE TEST:
*checking for the enzyme citrate permease that convert molecules in a cell to pyruvate
*3 citrate slants one with E.coli, E. aero, and 1 control uninoculated
with the needle we streaked the tops of the slants with zig-zag pattern
incubated for 24 hours
check for blue color change E. coli was green no color change -neg for citrate permease
E. aero changed blue +pos for enzyme activity

Oxidation-Fermentation test

OXIDATION FERMENTATION TEST:
*looking for aerobically & anaerobically or fermentaion of bacteria's ablility to utilize glucose
* we used hour glass shaped tubes with mineral oil seperating the middle so that oxygen cannot get to bottom. the medium is enriched with (O-F) high sugar-to-peptone ratio
its prepared with glucose, lactose, sucrose, maltose, mannitol, or xylose
*inoculated 3 with E. coli, P. aeru, A. faec with a needle and stabbed all the way 1/2 cm from the bottom of the butt. Incubated for 24 hrs.
the part that turns yellow is the oxidizor if turned blue its non-reactive it deaminated the peptone
if yellowe on top and bottom its a fermenter
E. coli-fermenter,  P. aeru is yellow only on top oxidizer, A. faec wuz non-reactive