Tuesday, July 24, 2012

Nitrate reduction test

NITRATE REDUCTION:
*to test for enzyme nitrate reductase
3 broths A. faecalis, E.coli, P. aerug
incubated for 24 hours
*look for gas bubble in all 3 broths- was present in A. faec +pos for NO - N2O - N2
*added reagent A & B to remaining broths- color change to red E. coli +pos for enzyme
next added zinc to remaining broth turned red P. aerug is -neg for enzyme





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