Tuesday, July 24, 2012

Citrate test

CITRATE TEST:
*checking for the enzyme citrate permease that convert molecules in a cell to pyruvate
*3 citrate slants one with E.coli, E. aero, and 1 control uninoculated
with the needle we streaked the tops of the slants with zig-zag pattern
incubated for 24 hours
check for blue color change E. coli was green no color change -neg for citrate permease
E. aero changed blue +pos for enzyme activity






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