Friday, July 27, 2012

Methyl Red & Voges Proskauer Test

METHYL RED: (M.R.)
*M.R. part of test is used to detect organisms capable of mixed acid fermentation.
-medium contains peptone & glucose with a phosphate buffer and pH indicator
Indicator: Methyl red dye
* the solution turned red with a +pos result and yellow is -neg.
VOGES- PROSKAUER: (V.P.)
*for organisms that can ferment glucose and convert acid products to acetoin & butanediol
we added vp reagents after incubation it turned red for pos+ result
we used E. coli & E. aero.
following incubation we transferred part broth into two other tubes for VP test
E. coli was +pos for MR & E. aero was +pos for VP.





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