Urea hydrolysis test

 UREA HYDROLYSIS TEST:

* test for enzyme urease
3 urea broths one uninoculated one for control, E.coli, P. vulg in the other 2 broths
incubate for 24 hours . coli
examined for color change. phenol red is a pH indicator used

the P. vulg was pink pH above 8.5 it turned pink +pos for urease
E. coli pH was low turned yellow -neg for enzyme