Tuesday, July 24, 2012

Tryptone test

TRYPTONE TEST:
*checking for the enzyme cysteine desulfurase a sulfur reduction enzyme. also thiosulfate reductases
for bacteria producing indole is SIM medium is used for. this test is a series of tests along with Indole, Methyl Red. Voges-Proskauer, and Citrate
*we stab inoculated 2 broths with a needle and incubated for 24 hours
we used E. coli & E. aero
*examined the stabline for spreading and black precipitation in the medium
the E. coli changed color and broke down indole to pyruvate is +pos for enzyme
the E. aero is -neg for enzyme no color change





No comments:

Post a Comment