Saturday, July 28, 2012

MacConkey Agar

MACCONKEY AGAR TEST:
*Medium contains lactose,bile salts, neutral red, and crystal violet.
-to grow and check gram- neg bacteria for lactose fermentation
inhibitor: bile salts & crystal violet inhibits gram +pos growth
pH indicator: neutral red dye
* lactose fermenters will turn the agar red or pink for a pos+ result
-we streaked 2 agar plates sarting in section 1-4 incubated for 24 hours
*E. coli had good growth-red is a lactose fermenter
*S. epi had poor growth no ferm.
*S. enter had good beige growth no lactose ferm
*E. aero good red growth- ferments lactose





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