Friday, July 27, 2012

Phenol Red Broth Test

PHENOL RED BROTH:
*used to detect organism's ability to ferment in after a carbohydrate is added to test.
-indicator: Phenol red dye
-pH rises color turns pink is deamination of perptone and a neg.- result for carb hydrolysis.
-pH lowers it turns yellow a pos+ result for mixed acid fermentation
*3 tubes were used with phenol broth & durham tube for detection of gas production.
-inoculated with 3 organisms./ 24 hrs incubate
- A. faec,-no fermentation remained red
 E. faec-pink broth for peptone usage
 E. coli-yellow broth with a bubble in tube +pos for ferm







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